Ricky Collins died in hospital after being found by police in Killamarsh, Derbyshire, at 10pm on 29 March.
Derbyshire Police said a 24-year-old man and a 29-year-old woman, both from Sheffield, were detained on Monday and remain in custody.
A 16-year-old boy, from Sheffield and held on suspicion of murder, has been bailed pending further inquiries.
Mr Collins, from Sheffield, was found injured after officers were called to reports of a disturbance at a house in Westthorpe Road.
He had sustained stab wounds and died later in hospital, the force said.
Martin Mongan, 44, of Park Estate, Pontefract, West Yorkshire, and a 43-year-old man from Sheffield have been charged with assisting an offender.
